{"id":2176,"date":"2023-09-20T10:01:40","date_gmt":"2023-09-20T01:01:40","guid":{"rendered":"https:\/\/www.elst.okayama-u.ac.jp\/en\/?page_id=2176"},"modified":"2026-08-07T11:15:05","modified_gmt":"2026-08-07T02:15:05","slug":"areas02_animal","status":"publish","type":"page","link":"https:\/\/www.elst.okayama-u.ac.jp\/en\/education\/mc-chikyu\/areas02_animal\/","title":{"rendered":"Animal Development and Reproductive Biotechnology"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-69 aligncenter\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/theme.png\" alt=\"\" width=\"418\" height=\"405\"><\/p>\n<p>We are developing new more effective IVF systems and micromanipulation techniques for gametes in mammals including humans. Through these technological developments, we are also undertaking basic studies to make clear the systems of gamete (oocytes and spermatozoa) formation, fertilization and early development, as well as applied studies to improve the efficiency in the production of more value-added useful animals. The details are as follows.<\/p>\n<table class=\"el_table_fix\" style=\"width: 100%\">\n<tbody>\n<tr>\n<th style=\"width: 20%\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/Hiroaki_FUNAHASHI.jfif\" width=\"320\" height=\"408\"><\/th>\n<td style=\"width: 80%\">\n<ul>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">Prof. Dr. FUNAHASHI Hiroaki<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">E-mail: hirofun <span style=\"color: #0000ff\">[at]<\/span> okayama-u.ac.jp<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">reproductive biology , ooctyte maturation, sperm capacitation, in vitro embryo production, mitochondrial quality control<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt\"><a class=\"el_linkBtn\" href=\"https:\/\/soran.cc.okayama-u.ac.jp\/html\/9633655de308791874506e4da22f6611_en.html\">Directory of Researchers<\/a>&nbsp;<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<th style=\"width: 20%\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/Takuya_WAKAI.jfif\" width=\"320\" height=\"408\"><\/th>\n<td style=\"width: 80%\">\n<ul>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">Assoc. Prof. Dr. WAKAI Takuya<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">E-mail: t2wakai <span style=\"color: #0000ff\">[at]<\/span> okayama-u.ac.jp<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">reproductive biology , ooctyte maturation, sperm capacitation, in vitro embryo production, mitochondrial quality control<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt\"><a class=\"el_linkBtn\" href=\"https:\/\/soran.cc.okayama-u.ac.jp\/html\/4f503d8d9448fa1b74506e4da22f6611_en.html\">Directory of Researchers<\/a>&nbsp;<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Development of new systems to produce embryos in vitro from oocytes collected from small diameter follicles<\/strong><\/p>\n<p><a href=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img01.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-thumbnail\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img01.jpg\" width=\"212\" height=\"160\"><\/a>For in vitro embryo production in mammals, especially domestic animals, usually oocyte-cumulus complexes derived from middle follicles with a diameter of 3-6 mm have been used. However, a large number of small follicles with less than 3 mm in diameter, rather than middle follicles, exist on the surface of ovaries. But the developmental competence of the complexes from small follicles (in meiosis and early development following fertilization) has been known to be much lower than those from middle follicles. We&#8217;re trying to make clear why the developmental competence is lower in the oocytes from small follicle as compared with middle ones and whether those abilities are improved when what kind of processing are applied.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>New assessment of sperm quality and the function associated with penetration<\/strong><\/p>\n<p><a href=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img02.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-thumbnail\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img02.jpg\" width=\"213\" height=\"160\"><\/a>In mammals, although a quite large number of spermatozoa are ejaculated into the female reproductive tract before ovulation, consequently only one spermatozoon will be able to penetrate into an ovulated oocyte. So far, sperm function and the quality has been assessed as the motility and membrane intactness of spermatozoa under a microscope. However, this assessment may not be ideal in objectivity and may require quite expensive equipment such as a flow-cytometer. So, we are focusing on the work of the mitochondria and RNA in spermatozoa to make clear the mechanisms regulating the function and maintaining the quality of spermatozoa. We are also applying the knowledge to sperm assessment.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Roles of organelles in mammalian oocytes<\/strong><\/p>\n<p><a href=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img03.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-thumbnail\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img03.jpg\" width=\"185\" height=\"185\"><\/a>Gametes (oocytes and spermatozoa) are highly specialized cells for the transmission of genes to the progeny. Our laboratory is interested in understanding the mechanisms of oocyte development, maturation and fertilization in mammals. Problems in oocyte quality cause the infertility, and the development of organelles, such as mitochondria and endoplasmic reticulum, is a key determinant for the successful fertilization and subsequent embryonic development. We are using several techniques to address the roles of organelles in oocytes including in vitro maturation, fertilization, genetic manipulation and live cell imaging.<\/p>\n<p>&nbsp;<\/p>\n<table class=\"el_table_fix\" style=\"width: 100%\">\n<tbody>\n<tr>\n<th style=\"width: 20%\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/ABE Kenichiro.jpg\" width=\"320\" height=\"408\"><\/th>\n<td style=\"width: 80%\">\n<ul>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">Asst.Prof. ABE Kenichiro<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">E-mail: k-abe<span id=\"content_ousdb1_lp$002fitem$005b2$005d$002fsys_mail\"><span style=\"color: #0000ff\">&nbsp;[at]<\/span> <\/span>okayama-u.ac.jp<\/span><\/li>\n<li style=\"list-style-type: none;font-size: 12px;margin-left: -20px\"><span style=\"font-size: 12pt\">Pre-implantation embryos,Gene regulation,Reprogramming,Reproductive biology,In vitro embryo production\n<p><\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt\"><a class=\"el_linkBtn\" href=\"https:\/\/soran.cc.okayama-u.ac.jp\/html\/2503c253806c597dbc16db0f9fd601f5_en.html\">Directory of Researchers<\/a>&nbsp;<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<div style=\"width: 276px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.elst.okayama-u.ac.jp\/up_load_files\/areas\/areas02_animal\/img_ABE Kenichiro.jpg\" alt=\"\" width=\"266\" height=\"203\"><p class=\"wp-caption-text\">Microinjection into porcine embryo<\/p><\/div>\n<p>Pre-implantation embryos possess the extraordinary ability to give rise to a complete organism, known as totipotency. To elucidate the molecular mechanisms underlying totipotency, we are investigating gene regulatory mechanisms in porcine and murine embryos. Notably, in vitro-produced embryos often exhibit low developmental competence due to compromised totipotency. By manipulating the gene regulatory mechanisms, we aim to establish advanced methodologies to restore totipotency and enhance the efficiency of animal production.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are developing new more effective IVF systems and micromanipulation techniques for gametes in mammals inclu [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":150,"menu_order":139,"comment_status":"closed","ping_status":"closed","template":"template-layout_single.php","meta":{"footnotes":""},"class_list":["post-2176","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.10 - aioseo.com -->\n\t<meta name=\"description\" content=\"We are developing new more effective IVF systems and mi\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<link rel=\"canonical\" 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